Additional file 2: of Mitochondrial damage and “plugging” of transport selectively in myelinated, small-diameter axons are major early events in peripheral neuroinflammation

Video S2. High-power time-lapse video of inflamed saphenous nerve on the first day of onset of EAN, taken 40 min after the exposure of saphenous nerve. Forty minutes following the first time-lapse video (Additional file 1: Video S1), the same axons are apparent in the field of view. Some mobile inflammatory cells (surface labeled with IB4+, green) and functional mitochondria (TMRM, red) can be seen. Notably, one inflammatory cell (encircled) can be seen closely juxtaposed to the same axon as seen 40 min earlier, still stationary. This is likely to be the same cell, remaining in the close proximity to the axon throughout the 40-min period. The accumulation of axonal mitochondria within this axon appear extended, with some loss of bright red TMRM labelling (mitochondrial membrane potential), suggesting possible mitochondrial damage in that area. (AVI 2879 kb